Porcine CD163 Recombinant Protein (RPPB4196)
- SKU:
- RPPB4196
Description
| Product Name: | Porcine CD163 Recombinant Protein |
| Product Code: | RPPB4196 |
| Size: | 50µg |
| Species: | Porcine |
| Target: | CD163 |
| Synonyms: | CD-163, Hemoglobin scavenger receptor, macrophage-associated antigen, M130, sCD163, CD163, MM130. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a 0.2�m filtered concentrated solution in 1�PBS, pH 7.4, containing 4M Urea. |
| Solubility: | It is recommended to reconstitute the lyophilized CD163 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized CD163 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD163 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MDKLRMVLHE NSGSADLKLR VVDGVTECSG RLEVKFQGEW GTICDDGWDS DDAAVACKQL GCPTAVTAIG RVNASEGTGH IWLDSVSCHG HESALWQCRH HEWGKHYCNH NEDAGVTCSD GSDLELRLKG GGSHCAGTVE VEIQKLVGKV CDRSWGLKEA DVVCRQLGCG SALKTSYQVY SKTKATNTWL FVSSCNGNET SLWDCKNWQW GGLSCDHYDE AKITCSAHRK PRLVGGDIPC SGRVEVQHGD TWGTVCDSDF SLEAASVLCR ELQCGTVVSL LGGAHFGEGS GQIWAEEFQC EGHESHLSLC PVAPRPDGTC SHSRDVGVVC SRYTQIRLVN GKTPCEGRVE LNILGSWGSL CNSHWDMEDA HVLCQQLKCG VALSIPGGAP FGKGSEQVWR HMFHCTGTEK HMGDCSVTAL GASLCSSGQV ASVICSGNQS QTLSPCNSSS SDPSSSIISE ENGVACIGSG QLRLVDGGGR CAGRVEVYHE GSWGTICDDS WDLNDAHVVC KQLSCGWAIN ATGSAHFGEG TGPIWLDEIN CNGKESHIWQ CHSHGWGRHN CRHKEDAGVI CSEFMSLRLI SENSRETCAG RLEVFYNGAW GSVGKNSMSP ATVGVVCRQL GCADRGDISP ASSDKTVSRH MWVDNVQCPK GPDTLWQCPS SPWKKRLASP SEETWITCAN KIRLQEGNTN CSGRVEIWYG GSWGTVCDDS WDLEDAQVVC RQLGCGSALE AGKEAAFGQG TGPIWLNEVK CKGNETSLWD CPARSWGHSD CGHKEDAAVT CSEIAKSRES LHATGRSHHH HHHHH |
CD163 is an acute phase-regulated receptor which participates in the removal and endocytosis of hemoglobin/haptoglobin complexes by macrophages and thus keeps tissues from free hemoglobin-mediated oxidative damage. Furthermore, CD163 partakes in the uptake and recycling of iron, through endocytosis of hemoglobin/haptoglobin and ensuing breakdown of heme. In addition, CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent way. CD163 demonstrates greater affinity for complexes of hemoglobin and multimeric haptoglobin of HP-1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP-1S phenotype. Moreover, CD163 stimulates a cascade of intracellular signals which involves tyrosine kinase-dependent calcium recruitment, inositol triphosphate formation and secretion of IL-6�& CSF-1.
CD163 Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 805 amino acids and having a molecular mass of 87kDa.The CD163 is fused to an 8 amino acid His Tag at C-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. May play a role in the process of infection of porcine monocytes/macrophages by African swine fever virus (ASFV). In case of porcine reproductive and respiratory syndrome virus (PRRSV), serves mediates virion attachment and plays a role in viral entry. |
| UniProt Code: | Q2VL90 |
| NCBI GenInfo Identifier: | 47522778 |
| NCBI Gene ID: | 397031 |
| NCBI Accession: | NP_999141.1 |
| UniProt Secondary Accession: | Q2VL90,Q2VL89, Q8HY22, |
| UniProt Related Accession: | Q2VL90 |
| Molecular Weight: | |
| NCBI Full Name: | scavenger receptor cysteine-rich type 1 protein M130 |
| NCBI Official Symbol: | CD163�� |
| NCBI Protein Information: | scavenger receptor cysteine-rich type 1 protein M130 |
| UniProt Protein Name: | Scavenger receptor cysteine-rich type 1 protein M130 |
| UniProt Synonym Protein Names: | CD_antigen: CD163Soluble CD163; sCD163 |
| Protein Family: | Scavenger receptor cysteine-rich type 1 protein |
| UniProt Gene Name: | CD163�� |
| UniProt Entry Name: | C163A_PIG |
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