Antibody Name:	POLR3E Antibody (PACO40882)
Antibody SKU:	PACO40882
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human DNA-directed RNA polymerase III subunit RPC5 protein (1-260AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Immunohistochemistry of paraffin-embedded human testis tissue using PACO40882 at dilution of 1:100.
	Immunohistochemistry of paraffin-embedded human skin tissue using PACO40882 at dilution of 1:100.

Background:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).

Synonyms:

DNA-directed RNA polymerase III subunit RPC5 (RNA polymerase III subunit C5) (DNA-directed RNA polymerase III 80 kDa polypeptide), POLR3E, KIAA1452

UniProt Protein Function:	POLR3E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription initiation complex; EC 2.7.7.6; TransferaseChromosomal Location of Human Ortholog: 16p12.2Cellular Component: cytosol; DNA-directed RNA polymerase III complex; nucleoplasmBiological Process: defense response to virus; gene expression; innate immune response; positive regulation of interferon type I production; RNA elongation from RNA polymerase III promoter; termination of RNA polymerase III transcription; transcription from RNA polymerase III promoter
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q9NVU0
NCBI GenInfo Identifier:	29428028
NCBI Gene ID:	101060521
NCBI Accession:	Q9NVU0.1
UniProt Secondary Accession:	Q9NVU0,Q9BWF7, Q9H8W8, Q9H907, Q9P276, B4DL24, B4DUP6 H3BT11
UniProt Related Accession:	Q9NVU0
Molecular Weight:	75,758 Da
NCBI Full Name:	DNA-directed RNA polymerase III subunit RPC5
NCBI Synonym Full Names:
NCBI Official Symbol:	LOC101060521
NCBI Official Synonym Symbols:
NCBI Protein Information:	DNA-directed RNA polymerase III subunit RPC5
UniProt Protein Name:	DNA-directed RNA polymerase III subunit RPC5
UniProt Synonym Protein Names:	DNA-directed RNA polymerase III 80 kDa polypeptide
Protein Family:	DNA-directed RNA polymerase
UniProt Gene Name:	POLR3E
UniProt Entry Name:	RPC5_HUMAN