Name: POLE Antibody (PACO22306)
SKU: PACO22306
Availability: OutOfStock

Antibody Name:	POLE Antibody (PACO22306)
Antibody SKU:	PACO22306
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:50-1:100
Species Reactivity:	Human, Mouse
Immunogen:	Synthesized peptide derived from internal of human POLE1.

Form:	Liquid
Storage Buffer:	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using POLE1 antibody.

Background:	Participates in DNA repair and in chromosomal DNA replication.
Synonyms:	DNA polymerase epsilon catalytic subunit A; EC 2.7.7.7; DNA polymerase II subunit A;

UniProt Protein Function:	POLE: Participates in DNA repair and in chromosomal DNA replication. Belongs to the DNA polymerase type-B family.Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 2.7.7.7; DNA replication; DNA repair, damage; TransferaseChromosomal Location of Human Ortholog: 12q24.3Cellular Component: nucleoplasm; epsilon DNA polymerase complex; cytoplasm; plasma membrane; nucleusMolecular Function: DNA binding; zinc ion binding; 4 iron, 4 sulfur cluster binding; nucleotide binding; DNA-directed DNA polymerase activity; chromatin bindingBiological Process: DNA replication initiation; in utero embryonic development; DNA repair; base-excision repair, gap-filling; telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycleDisease: Colorectal Cancer, Susceptibility To, 12; Facial Dysmorphism, Immunodeficiency, Livedo, And Short Stature
UniProt Protein Details:
NCBI Summary:	This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013]
UniProt Code:	Q07864
NCBI GenInfo Identifier:	62198237
NCBI Gene ID:	5426
NCBI Accession:	NP_006222.2
UniProt Secondary Accession:	Q07864,Q13533, Q86VH9
UniProt Related Accession:	Q07864
Molecular Weight:	261,518 Da
NCBI Full Name:	DNA polymerase epsilon catalytic subunit A
NCBI Synonym Full Names:	polymerase (DNA directed), epsilon, catalytic subunit
NCBI Official Symbol:	POLE
NCBI Official Synonym Symbols:	FILS; POLE1; CRCS12
NCBI Protein Information:	DNA polymerase epsilon catalytic subunit A
UniProt Protein Name:	DNA polymerase epsilon catalytic subunit A
UniProt Synonym Protein Names:	DNA polymerase II subunit A
Protein Family:	Mycosin
UniProt Gene Name:	POLE
UniProt Entry Name:	DPOE1_HUMAN