PNPLA5 Antibody (PACO58248)
- SKU:
- PACO58248
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | PNPLA5 Antibody (PACO58248) |
| Antibody SKU: | PACO58248 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Patatin-like phospholipase domain-containing protein 5 protein (90-205AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Colo320 whole cell lysate, A549 whole cell lysate. All lanes: PNPLA5 antibody at 3.1µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48, 36 kDa. Observed band size: 48 kDa. |
 | IHC image of PACO58248 diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO58248 diluted at 1:500 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Lipid hydrolase. |
| Synonyms: | Patatin-like phospholipase domain-containing protein 5 (EC 3.1.1) (GS2-like protein), PNPLA5, GS2L |
| UniProt Protein Function: | PNPLA5: Lipid hydrolase. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Hydrolase; EC 3.1.1.-Chromosomal Location of Human Ortholog: 22q13.31Cellular Component: cytosolMolecular Function: triacylglycerol lipase activityBiological Process: cellular lipid metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | This gene is a member of the patatin-like phospholipase family; its encoded protein has been shown to inhibit transacylation. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2010] |
| UniProt Code: | Q7Z6Z6 |
| NCBI GenInfo Identifier: | 74762442 |
| NCBI Gene ID: | 150379 |
| NCBI Accession: | Q7Z6Z6.1 |
| UniProt Secondary Accession: | Q7Z6Z6,Q6ZST0, B1AHL8, B3KPR1 |
| UniProt Related Accession: | Q7Z6Z6 |
| Molecular Weight: | 35,168 Da |
| NCBI Full Name: | Patatin-like phospholipase domain-containing protein 5 |
| NCBI Synonym Full Names: | patatin like phospholipase domain containing 5 |
| NCBI Official Symbol: | PNPLA5 |
| NCBI Official Synonym Symbols: | GS2L; dJ388M5; dJ388M5.4 |
| NCBI Protein Information: | patatin-like phospholipase domain-containing protein 5 |
| UniProt Protein Name: | Patatin-like phospholipase domain-containing protein 5 |
| UniProt Synonym Protein Names: | GS2-like protein |
| Protein Family: | Patatin-like phospholipase domain-containing protein |
| UniProt Gene Name: | PNPLA5 |
| UniProt Entry Name: | PLPL5_HUMAN |
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