PNCK Antibody (PACO61029)
- SKU:
- PACO61029
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | PNCK Antibody (PACO61029) |
| Antibody SKU: | PACO61029 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant Human Calcium/calmodulin-dependent protein kinase type 1B protein (1-115AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Mouse brain tissue, Mouse kidney tissue. All lanes: PNCK antibody at 3.1µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39, 41, 28, 47 kDa. Observed band size: 39 kDa. |
 | IHC image of PACO61029 diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 (By similarity). |
| Synonyms: | Calcium/calmodulin-dependent protein kinase type 1B (EC 2.7.11.17) (CaM kinase I beta) (CaM kinase IB) (CaM-KI beta) (CaMKI-beta) (Pregnancy up-regulated non-ubiquitously-expressed CaM kinase), PNCK |
| UniProt Protein Function: | CAMK1B: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; CAMK group; CAMK1 family Chromosomal Location of Human Ortholog: Xq28 |
| NCBI Summary: | PNCK is a member of the calcium/calmodulin-dependent protein kinase family of protein serine/threonine kinases (see CAMK1; MIM 604998) (Gardner et al., 2000 [PubMed 10673339]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q6P2M8 |
| NCBI GenInfo Identifier: | 67466915 |
| NCBI Gene ID: | 139728 |
| NCBI Accession: | Q6P2M8.2 |
| UniProt Secondary Accession: | Q6P2M8,Q8N4R0, B4DJR8, B4E1A6, B7WPG0, D3DWU7, |
| UniProt Related Accession: | Q6P2M8 |
| Molecular Weight: | 40,356 Da |
| NCBI Full Name: | Calcium/calmodulin-dependent protein kinase type 1B |
| NCBI Synonym Full Names: | pregnancy up-regulated nonubiquitous CaM kinase |
| NCBI Official Symbol: | PNCK |
| NCBI Official Synonym Symbols: | BSTK3; CaMK1b |
| NCBI Protein Information: | calcium/calmodulin-dependent protein kinase type 1B |
| UniProt Protein Name: | Calcium/calmodulin-dependent protein kinase type 1B |
| UniProt Synonym Protein Names: | CaM kinase I beta; CaM kinase IB; CaM-KI beta; CaMKI-beta; Pregnancy up-regulated non-ubiquitously-expressed CaM kinase |
| Protein Family: | Calcium/calmodulin-dependent protein kinase |
| UniProt Gene Name: | PNCK |
| UniProt Entry Name: | KCC1B_HUMAN |
| Antibodies | ELISA Kits |
| PNCK Antibody (PACO03503) | CaMK1-beta Colorimetric Cell-Based ELISA |
| PNCK Antibody (PACO11324) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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