PLA2G4F Antibody (PACO63107)
- SKU:
- PACO63107
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | PLA2G4F Antibody (PACO63107) |
| Antibody SKU: | PACO63107 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Cytosolic phospholipase A2 zeta protein (74-299AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO63107 diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine (By similarity). |
| Synonyms: | Cytosolic phospholipase A2 zeta (cPLA2-zeta) (EC 3.1.1.4) (Phospholipase A2 group IVF), PLA2G4F |
| UniProt Protein Function: | PLA2G4F: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine. Has a much higher lysophospholipase and PLA(2) activity than PLA2G4B. Potently inhibited by pyrrolidine-2 and Wyeth-1. Its specific activity, inhibitor sensitivity, and low micromolar calcium dependence are similar to PLA2G4A. Translocates to ruffles and dynamic vesicular structures in a calcium-dependent manner. The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ Three isoforms of the human protein are produced by alternative splicing.Protein type: Lipid Metabolism - arachidonic acid; EC 3.1.1.4; PhospholipaseChromosomal Location of Human Ortholog: 15q15.1Cellular Component: cytosolMolecular Function: lysophospholipase activity; phospholipase A1 activity; phospholipase A2 activityBiological Process: phospholipid metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q68DD2 |
| NCBI GenInfo Identifier: | 317373489 |
| NCBI Gene ID: | 255189 |
| NCBI Accession: | Q68DD2.3 |
| UniProt Secondary Accession: | Q68DD2,Q6ZMC8 |
| UniProt Related Accession: | Q68DD2 |
| Molecular Weight: | 95,310 Da |
| NCBI Full Name: | Cytosolic phospholipase A2 zeta |
| NCBI Synonym Full Names: | phospholipase A2 group IVF |
| NCBI Official Symbol: | PLA2G4F |
| NCBI Official Synonym Symbols: | PLA2G4FZ |
| NCBI Protein Information: | cytosolic phospholipase A2 zeta |
| UniProt Protein Name: | Cytosolic phospholipase A2 zeta |
| UniProt Synonym Protein Names: | Phospholipase A2 group IVF |
| Protein Family: | Cytosolic phospholipase |
| UniProt Gene Name: | PLA2G4F |
| UniProt Entry Name: | PA24F_HUMAN |
| Antibodies |
| PLA2G4F Antibody (PACO11281) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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