Name: PLA2G4E Antibody (PACO63419)
SKU: PACO63419
Availability: OutOfStock

Antibody Name:	PLA2G4E Antibody (PACO63419)
Antibody SKU:	PACO63419
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Cytosolic phospholipase A2 epsilon protein (604-868AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO63419 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with PACO63419 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:	Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position.
Synonyms:	Cytosolic phospholipase A2 epsilon (cPLA2-epsilon) (EC 3.1.1.4) (Phospholipase A2 group IVE), PLA2G4E

UniProt Protein Function:	PLA2G4E: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes glycerophospholipids in the sn-2 position. Translocates to lysosomal membranes in a calcium-dependent fashion. The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Two isoforms of the human protein are produced by alternative splicing.Protein type: Lipid Metabolism - alpha-linolenic acid; Phospholipase; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4Chromosomal Location of Human Ortholog: 15q15.1Cellular Component: lysosomal membrane; cytosolMolecular Function: phospholipase A2 activity; metal ion bindingBiological Process: phospholipid catabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q3MJ16
NCBI GenInfo Identifier:	331284217
NCBI Gene ID:	123745
NCBI Accession:	NP_001193599.1
UniProt Secondary Accession:	Q3MJ16,Q6ZSC0
UniProt Related Accession:	Q3MJ16
Molecular Weight:	56,715 Da
NCBI Full Name:	cytosolic phospholipase A2 epsilon
NCBI Synonym Full Names:	phospholipase A2, group IVE
NCBI Official Symbol:	PLA2G4E
NCBI Official Synonym Symbols:
NCBI Protein Information:	cytosolic phospholipase A2 epsilon; cPLA2-epsilon
UniProt Protein Name:	Cytosolic phospholipase A2 epsilon
UniProt Synonym Protein Names:	Phospholipase A2 group IVE
Protein Family:	Cytosolic phospholipase
UniProt Gene Name:	PLA2G4E
UniProt Entry Name:	PA24E_HUMAN