Antibody Name:	PLA2G4B Antibody (PACO62959)
Antibody SKU:	PACO62959
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Cytosolic phospholipase A2 β protein (514-781AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate. All lanes: P0C869 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 88, 101, 55, 115 kDa. Observed band size: 88 kDa.
	Immunofluorescence staining of HepG2 cells with PACO62959 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO62959 diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A. Isoform 3 has calcium-dependent activity against palmitoyl-arachidonyl-phosphatidylethanolamine and low level lysophospholipase activity but no activity against phosphatidylcholine. Isoform 5 does have activity against phosphatidylcholine.

Synonyms:

Cytosolic phospholipase A2 β (cPLA2-beta) (EC 3.1.1.4) (Phospholipase A2 group IVB), PLA2G4B

UniProt Protein Function:	PLA2G4B: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A. Not inhibited by pyrrolidine-2 and Wyeth-1. The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Four isoforms of the human protein are produced by alternative splicing. Isoform 3 translocates to membrane vesicles in a calcium-dependent fashion and has activity against palmitoyl-arachidonyl-phosphatidylethanolamine and low level lysophospholipase activity but no activity against phosphatidylcholine.Protein type: Phospholipase; EC 3.1.1.4; Lipid Metabolism - arachidonic acidChromosomal Location of Human Ortholog: 15q11.2-q21.3Cellular Component: cytosol; extracellular region; mitochondrial inner membraneMolecular Function: calcium-dependent phospholipase A2 activity; lysophospholipase activity; phospholipase A1 activity; phospholipase A2 activityBiological Process: glycerophospholipid catabolic process; phosphatidic acid biosynthetic process; phospholipid metabolic process
UniProt Protein Details:
NCBI Summary:	This gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acids. This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains. [provided by RefSeq, Jul 2008]
UniProt Code:	P0C869
NCBI GenInfo Identifier:	300669659
NCBI Gene ID:	100137049
NCBI Accession:	P0C869.2
UniProt Secondary Accession:	P0C869,O95712, Q19KD5, Q19KD6, Q59GF9, Q8TB10, Q9UKV7 B4DRT9
UniProt Related Accession:	P0C869
Molecular Weight:	114,121 Da
NCBI Full Name:	Cytosolic phospholipase A2 beta
NCBI Synonym Full Names:	phospholipase A2 group IVB
NCBI Official Symbol:	PLA2G4B
NCBI Official Synonym Symbols:	HsT16992; cPLA2-beta
NCBI Protein Information:	cytosolic phospholipase A2 beta
UniProt Protein Name:	Cytosolic phospholipase A2 beta
UniProt Synonym Protein Names:	Phospholipase A2 group IVB
Protein Family:	Cytosolic phospholipase
UniProt Gene Name:	PLA2G4B
UniProt Entry Name:	PA24B_HUMAN