Antibody Name:	PLA1A Antibody (PACO57920)
Antibody SKU:	PACO57920
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human Phospholipase A1 member A protein (293-398AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: HepG2 whole cell lysate. All lanes: PLA1A antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 50, 41, 48, 32 kDa. Observed band size: 50 kDa.
	IHC image of PACO57920 diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO57920 diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid, (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production.

Synonyms:

Phospholipase A1 member A (EC 3.1.1) (Phosphatidylserine-specific phospholipase A1) (PS-PLA1), PLA1A, NMD PSPLA1

UniProt Protein Function:	PLA1A: Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1- acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production. Belongs to the AB hydrolase superfamily. Lipase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted, signal peptide; EC 3.1.1.-; Secreted; Phospholipase Chromosomal Location of Human Ortholog: 3q13.13-q13.2 Cellular Component: acrosomal membrane Molecular Function:phospholipase A1 activity Biological Process: lipid catabolic process; lipid metabolic process; phosphatidylserine metabolic process
NCBI Summary:	The protein encoded by this gene is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine in liposomes. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]
UniProt Code:	Q53H76
NCBI GenInfo Identifier:	124015212
NCBI Gene ID:	51365
NCBI Accession:	Q53H76.2
UniProt Secondary Accession:	Q53H76,O95991, Q86WX6, Q9UPD2, B2R8V2, B4DXA2,
UniProt Related Accession:	Q53H76
Molecular Weight:	31,285 Da
NCBI Full Name:	Phospholipase A1 member A
NCBI Synonym Full Names:	phospholipase A1 member A
NCBI Official Symbol:	PLA1A
NCBI Official Synonym Symbols:	PSPLA1; PS-PLA1
NCBI Protein Information:	phospholipase A1 member A
UniProt Protein Name:	Phospholipase A1 member A
UniProt Synonym Protein Names:	Phosphatidylserine-specific phospholipase A1; PS-PLA1
Protein Family:	Phospholipase
UniProt Gene Name:	PLA1A
UniProt Entry Name:	PLA1A_HUMAN