PIRT Antibody (PACO60925)
- SKU:
- PACO60925
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | PIRT Antibody (PACO60925) |
| Antibody SKU: | PACO60925 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Phosphoinositide-interacting protein (1-55AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO60925 diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with PACO60925 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO60925 diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Regulatory subunit of TRPV1, a molecular sensor of noxious heat and capsaicin. Positively regulates TRPV1 channel activity via phosphatidylinositol 4,5-bisphosphate (PIP2). Binds various phosphoinositide, including phosphatidylinositol 4,5-bisphosphate (PIP2), but not phosphatidylinositol (PI) (By similarity). |
| Synonyms: | Phosphoinositide-interacting protein, PIRT |
| UniProt Protein Function: | PIRT: Regulatory subunit of TRPV1, a molecular sensor of noxious heat and capsaicin. Positively regulates TRPV1 channel activity via phosphatidylinositol 4,5-bisphosphate (PIP2). Binds various phosphoinositide, including phosphatidylinositol 4,5- bisphosphate (PIP2), but not phosphatidylinositol (PI).Protein type: Membrane protein, integral; Membrane protein, multi-passChromosomal Location of Human Ortholog: 17p12Cellular Component: integral to membrane |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P0C851 |
| NCBI GenInfo Identifier: | 157426881 |
| NCBI Gene ID: | 644139 |
| NCBI Accession: | NP_001094857.1 |
| UniProt Secondary Accession: | P0C851,B7Z648 |
| UniProt Related Accession: | P0C851 |
| Molecular Weight: | 15,334 Da |
| NCBI Full Name: | phosphoinositide-interacting protein |
| NCBI Synonym Full Names: | phosphoinositide-interacting regulator of transient receptor potential channels |
| NCBI Official Symbol: | PIRT |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | phosphoinositide-interacting protein |
| UniProt Protein Name: | Phosphoinositide-interacting protein |
| UniProt Synonym Protein Names: | |
| Protein Family: | Phosphoinositide-interacting protein |
| UniProt Gene Name: | PIRT |
| UniProt Entry Name: | PIRT_HUMAN |
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