Description
Product Name: | PIN1 Polyclonal Antibody |
Product Code: | CAB20718 |
Reactivity: | Arabidopsis thaliana |
Applications: | Western blotting |
Host Species: | Rabbit |
Purification Method: | Affinity purification |
Isotype: | IgG |
Reactivity: | Arabidopsis thaliana |
Tested Applications: | WB, ELISA |
Key Applications: | Western blotting |
Recommended Dilution: | WB 1:500-1:1000 |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
Positive Samples: | Inflorescences |
Cellular Location: | basal plasma membrane, cytoplasm, endoplasmic reticulum, plant-type cell wall, plasma membrane, plasmodesma |
Encodes an auxin efflux carrier involved in shoot and root development. It is involved in the maintenance of embryonic auxin gradients. Loss of function severely affects organ initiation, pin1 mutants are characterised by an inflorescence meristem that does not initiate any flowers, resulting in the formation of a naked inflorescence stem. PIN1 is involved in the determination of leaf shape by actively promoting development of leaf margin serrations. In roots, the protein mainly resides at the basal end of the vascular cells, but weak signals can be detected in the epidermis and the cortex. Expression levels and polarity of this auxin efflux carrier change during primordium development suggesting that cycles of auxin build-up and depletion accompany, and may direct, different stages of primordium development. PIN1 action on plant development does not strictly require function of PGP1 and PGP19 proteins.
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 391-480 of arabidopsis thaliana PIN1 (NP_177500.1). |
Sequence: | HADYS TATND HQKDV KISVP QGNSN DNQYV EREEF SFGNK DDDSK VLATD GGNNI SNKTT QAKVM PPTSV MTRLI LIMVW RKLIR NPNSY |
Synonyms: | ARABIDOPSIS THALIANA PIN-FORMED 1, ATPIN1, F6D5.2, F6D5_2, PIN-FORMED 1 |
Calculated MW: | 67kDa |
Observed MW: | 67kDa |