Signaling Pathway Assays

Phospholipase D Activity Assay Kit (BA0144)

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SKU:: BA0144
UPC:
MPN:
Product Type:: Assay
Instrument:: Microplate Reader
Sample Type:: Biological
Research Area:: Lipid Metabolism
Research Area:: Plant & Environmental Stress
Research Area:: Enzyme Activity
Research Area:: Clinical Chemistry

Description
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Description

Phospholipase D Activity Assay

Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers.

Assay Genie's phospholipase D activity assay provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H₂O₂ specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.

Applications

For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.

Phospholipase D Activity Assay - Key Features

Sensitive. Use 10 uL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.
Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening.

Kit Includes:	Assay Buffer: 10 mL, Dye Reagent: 120 mL, Enzyme Mix: Dried Substrate: 1.5 mL, Calibrator: 400 mL
Kit Requires:	Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader
Method of Detection:	OD570nm, or FL530/585nm
Detection Limit:	0.04 U/L
Samples:	Biological
Species:	All
Protocol Length:	30 min
Size:	100 tests
Storage:	Store all components at -20°C
Shelf Life:	6 months