Phosphofructokinase（PFK）Activity Assay Kit
- SKU:
- MAES0262
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Serum, Plasma, Tissue And Cell Sample
- Sensitivity:
- 0.27 U/L
- Range:
- 0.27-32.29 U/L
- Assay Time:
- 15 min
Description
| Product Name: | Phosphofructokinase（PFK）Activity Assay Kit |
| Product Code: | MAES0262 |
| Sample Type: | Serum, Plasma, Tissue And Cell Sample |
| Assay Time: | 15 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | PFK |
| Detection Instrument: | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 0.27 U/L |
| Detection Range: | 0.27-32.29 U/L |
| Inter-assay CV: | 0.08 |
| Intra-assay CV: | 0.03 |
Phosphofructokinase(also known as 6-phosphofructokinase; PFK) is a class of kinases that act on fructose-6-phosphate. Phosphofructokinase catalyzes fructose-6-phosphate and ATP to produce fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. The activity of PFK can be reflected by the determination of NADH decline rate at 340 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Normal saline (0.9% NaCl) |
| Other Instruments Required: | Centrifuge |
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