Phospho-SYT1/SYT2 (Thr202/199) Antibody (PACO23871)
- SKU:
- PACO23871
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | Phospho-SYT1/SYT2 (Thr202/199) Antibody (PACO23871) |
| Antibody SKU: | PACO23871 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:100-1:200 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Peptide sequence around phosphorylation site of threonine 202/199 (R-K-T(p)-L-N) derived from Human Synaptotagmin 1/2. |
| Form: | Liquid |
| Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of methanol-fixed Hela cells using Synaptotagmin 1/2 (Phospho-Thr202/199) Antibody. |
| Background: | The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse |
| Synonyms: | SYT1/2; SytI/II; Synaptotagmin I/II; P65; SYT |
| UniProt Protein Function: | SYT1: an integral membrane protein of synaptic vesicles thought to serve as a Ca(2+) sensor in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse. Binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Vesicle; Calcium-binding Chromosomal Location of Human Ortholog: 12cen-q21 Cellular Component: neuron projection; plasma membrane; synaptic vesicle; synaptic vesicle membrane Molecular Function:calcium ion binding; calcium-dependent phospholipid binding; clathrin binding; low-density lipoprotein receptor binding; phosphatidylinositol binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; SNARE binding; syntaxin-1 binding Biological Process: calcium ion-dependent exocytosis of neurotransmitter; detection of calcium ion; glutamate secretion; neurotransmitter secretion; positive regulation of synaptic transmission; protein homooligomerization; regulation of calcium ion-dependent exocytosis; regulation of exocytosis; regulation of synaptic transmission, glutamatergic; synaptic transmission; synaptic vesicle endocytosis; vesicle fusion |
| NCBI Summary: | The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010] |
| UniProt Code: | P21579 |
| NCBI GenInfo Identifier: | 135086 |
| NCBI Gene ID: | 6857 |
| NCBI Accession: | P21579.1 |
| UniProt Secondary Accession: | P21579,Q6AI31, |
| UniProt Related Accession: | P21579 |
| Molecular Weight: | 47,573 Da |
| NCBI Full Name: | Synaptotagmin-1 |
| NCBI Synonym Full Names: | synaptotagmin 1 |
| NCBI Official Symbol: | SYT1 |
| NCBI Official Synonym Symbols: | P65; SYT; SVP65 |
| NCBI Protein Information: | synaptotagmin-1 |
| UniProt Protein Name: | Synaptotagmin-1 |
| UniProt Synonym Protein Names: | Synaptotagmin I; SytI; p65 |
| Protein Family: | Synaptotagmin |
| UniProt Gene Name: | SYT1 |
| UniProt Entry Name: | SYT1_HUMAN |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
