Antibody Name:	Phospho-PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCQ/PRKCZ (T497) Antibody
Antibody SKU:	PACO03002
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000
Species Reactivity:	Human, Mouse, Rat
Immunogen:	synthesized peptide derived from human PKC around the phosphorylation site of T497.

Form:	Liquid
Storage Buffer:	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Synonyms:

PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCB; PKCB; PRKCB1; Protein kinase C beta type; PKC-B; PKC-beta; PRKCD; Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; PRKCE; PKCE; Protein kinase

UniProt Protein Function:	PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators.
UniProt Protein Details:	Protein type:EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Oncoprotein; Kinase, protein; Protein kinase, AGC; AGC group; PKC family; Alpha subfamily Chromosomal Location of Human Ortholog: 17q22-q23.2 Cellular Component: nucleoplasm; photoreceptor outer segment; mitochondrion; cell soma; endoplasmic reticulum; perinuclear region of cytoplasm; apical part of cell; mitochondrial membrane; dendrite; cytoplasm; plasma membrane; cytosol Molecular Function:protein serine/threonine kinase activity; protein binding; enzyme binding; protein kinase C activity; zinc ion binding; calcium-dependent protein kinase C activity; ATP binding; protein kinase activity Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; positive regulation of cell adhesion; nerve growth factor receptor signaling pathway; regulation of muscle contraction; mitotic nuclear envelope disassembly; positive regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of insulin receptor signaling pathway; signal transduction; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; induction of positive chemotaxis; synaptic transmission; negative regulation of cell proliferation; chondrocyte differentiation; angiogenesis; cell adhesion; positive regulation of macrophage differentiation; regulation of peptidyl-tyrosine phosphorylation; regulation of the force of heart contraction; inactivation of MAPK activity; epidermal growth factor receptor signaling pathway; platelet activation; neutrophil chemotaxis; fibroblast growth factor receptor signaling pathway; positive regulation of blood vessel endothelial cell migration; adenylate cyclase activation; negative regulation of adenylate cyclase activity; cellular calcium ion homeostasis; rhodopsin mediated signaling; negative regulation of glucose import; positive regulation of angiogenesis; regulation of rhodopsin mediated signaling; phospholipase C activation; energy reserve metabolic process; innate immune response; gene expression; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; blood coagulation; vascular endothelial growth factor receptor signaling pathway; regulation of insulin secretion; positive regulation of cell migration; positive regulation of inflammatory response
NCBI Summary:	Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]
UniProt Code:	P17252
NCBI GenInfo Identifier:	317373571
NCBI Gene ID:	5578
NCBI Accession:	P17252.4
UniProt Secondary Accession:	P17252,Q15137, Q32M72, Q96RE4, B5BU22,
UniProt Related Accession:	P17252
Molecular Weight:	76,750 Da
NCBI Full Name:	Protein kinase C alpha type
NCBI Synonym Full Names:	protein kinase C, alpha
NCBI Official Symbol:	PRKCA
NCBI Official Synonym Symbols:	AAG6; PKCA; PRKACA; PKC-alpha
NCBI Protein Information:	protein kinase C alpha type; PKC-A; aging-associated gene 6
UniProt Protein Name:	Protein kinase C alpha type
Protein Family:	Protein kinase
UniProt Gene Name:	PRKCA
UniProt Entry Name:	KPCA_HUMAN