Phospho-POLR2A-S2/S5 Monoclonal Antibody
- SKU:
- CABP1354
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- Applications:
- IP
Description
| Product Name: | Phospho-POLR2A-S2/S5 Monoclonal Antibody |
| Product Code: | CABP1354 |
| Reactivity: | Human, Rat |
| Applications: | Western blotting, Immunoprecipitation |
| Host Species: | Rabbit |
| Purification Method: | Affinity purification |
| Isotype: | IgG |
| Clone No: | ARC54959 |
| Reactivity: | Human, Rat |
| Tested Applications: | WB, IP, ChIP, ELISA, CUT&Tag |
| Key Applications: | Western blotting, Immunoprecipitation |
| Recommended Dilution: | WB 1:1000-1:5000, IP 1:2000-1:6000, ChIP 1:50-1:200, CUT&Tag 10, ⁵ cells /1 μg |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Positive Samples: | HeLa |
| Cellular Location: | Nucleus |
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S2/S5 of human POLR2A. |
| Sequence: | Email for sequence |
| Synonyms: | RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, NEDHIB, RpIILS, hsRPB1, hRPB220 |
| Calculated MW: | 217kDa |
| Observed MW: | 270kDa |
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