Name: Phospho-Map2k5 (Ser311/Thr315) Antibody (PACO23912)
SKU: PACO23912
Availability: OutOfStock

Antibody Name:	Phospho-Map2k5 (Ser311/Thr315) Antibody (PACO23912)
Antibody SKU:	PACO23912
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Peptide sequence around phosphorylation site of Serine 311 and threonine 315 (V-N-S(p)I-A-K-T(p)-Y-V) derived from Rat MEK5.

Form:	Liquid
Storage Buffer:	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western blot analysis of extracts from Rat and Mouse brain tissue using MEK5(phospho-Ser311/ Thr315) Antibody.

Background:	Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
Synonyms:	Map2k5

UniProt Protein Function:	MEK5: a dual specificity protein kinase of the STE7 family. Activates ERK5 and is itself phosphorylated and activated by MEKK3, as well as by atypical protein kinase C isoforms. Involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced isoforms have been described.Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 familyCellular Component: membrane; spindle; intracellular; nucleus; cytosolMolecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; metal ion binding; protein-tyrosine kinase activity; ATP binding; protein kinase activityBiological Process: peptidyl-tyrosine phosphorylation; negative regulation of heterotypic cell-cell adhesion; activation of MAPK activity; heart development; MAPKKK cascade; negative regulation of interleukin-8 biosynthetic process; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; positive regulation of protein metabolic process; positive regulation of cell growth; inhibition of NF-kappaB transcription factor; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation
UniProt Protein Details:
NCBI Summary:	kinase involved in signal transduction in the cell [RGD, Feb 2006]
UniProt Code:	Q62862
NCBI GenInfo Identifier:	2499634
NCBI Gene ID:	29568
NCBI Accession:	Q62862.1
UniProt Secondary Accession:	Q62862,Q62863, Q62864
UniProt Related Accession:	Q62862
Molecular Weight:	448
NCBI Full Name:	Dual specificity mitogen-activated protein kinase kinase 5
NCBI Synonym Full Names:	mitogen activated protein kinase kinase 5
NCBI Official Symbol:	Map2k5
NCBI Official Synonym Symbols:	Mek5
NCBI Protein Information:	dual specificity mitogen-activated protein kinase kinase 5; MEK 5; MAPKK 5; MAPK/ERK kinase 5; MAP kinase kinase 5
UniProt Protein Name:	Dual specificity mitogen-activated protein kinase kinase 5
UniProt Synonym Protein Names:	MAPK/ERK kinase 5; MEK 5
Protein Family:	Dual specificity mitogen-activated protein kinase kinase
UniProt Gene Name:	Map2k5
UniProt Entry Name:	MP2K5_RAT