Phospho-Integrin Beta1 (Tyr795) In-Cell ELISA

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SKU:
CBCAB01701
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Cell Biology
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Detection Method:
Colorimetric

Description

Technical Manual

Phospho-Integrin Beta1 (Tyr795) Cell-Based ELISA

Product Description:The Phospho-Integrin Beta1 (Tyr795) Colorimetric Cell-Based ELISA Kit allows for qualitative detection of Phospho-IntegrinBeta1(Tyr795) in plated and fixed cells.
Target Synonyms:CD29; Fibronectin receptor beta subunit; FNRB; integrin beta-1; Integrin beta-1 precursor; Integrin VLA-4 beta subunit; ITB1
Detection Target:Phospho-IRAK1(Thr209)
Reactivity:H:Y795, M:Y795, R:Y796
Pack Size:2 x 96 assays
Detection Method:Colorimetric 450 nm
Format:Cell-Based ELISA/In-Cell ELISA
Storage and Stability Guarantee:4 °C/6 Months
Sample Type:Plated & fixed cells
Range:5000 cells/well minimum
Time:4.5 hours

Phospho-Integrin Beta1 (Tyr795) Cell-Based ELISA Protein Information

UniProt Protein Function:ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin.. Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).
UniProt Protein Details:

Protein type:Cell adhesion; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 10p11.22

Cellular Component: cell surface; cell-cell adherens junction; cytoplasm; filopodium; focal adhesion; lipid raft; membrane; neuromuscular junction; plasma membrane; receptor complex; ruffle; sarcolemma

Molecular Function:actin binding; C-X3-C chemokine binding; cell adhesion molecule binding; coreceptor activity; fibronectin binding; protease binding; protein binding; protein complex binding; protein heterodimerization activity

Biological Process: B cell differentiation; calcium-independent cell-matrix adhesion; cell adhesion mediated by integrin; cell migration; cell-cell adhesion mediated by integrin; cell-matrix adhesion; cell-substrate adhesion; cellular defense response; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; homophilic cell adhesion; integrin-mediated signaling pathway; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; mesodermal cell differentiation; positive regulation of apoptosis; positive regulation of GTPase activity; receptor internalization; regulation of immune response; stress fiber formation; transforming growth factor beta receptor signaling pathway

NCBI Summary:Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P05556
NCBI GenInfo Identifier:218563324
NCBI Gene ID:3688
NCBI Accession:P05556.2
UniProt Secondary Accession:P05556,P78466, P78467, Q13089, Q13090, Q13091, Q13212 A8K6N2, D3DRX9, D3DRY3, D3DRY4, D3DRY5,
UniProt Related Accession:P05556
Molecular Weight:88,884 Da
NCBI Full Name:Integrin beta-1
NCBI Synonym Full Names:integrin subunit beta 1
NCBI Official Symbol:ITGB1
NCBI Official Synonym Symbols:CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
NCBI Protein Information:integrin beta-1
UniProt Protein Name:Integrin beta-1
UniProt Synonym Protein Names:Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta; CD_antigen: CD29
UniProt Gene Name:ITGB1
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