Name: Phospho-HIST1H1B (T154) Antibody (PACO56601)
SKU: PACO56601
Availability: OutOfStock

Antibody Name:	Phospho-HIST1H1B (T154) Antibody (PACO56601)
Antibody SKU:	PACO56601
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IF, ChIP
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:200-1:2000, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Peptide sequence around site of Phospho-Thr (154) derived from Human Histone H1.5

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate. All lanes: HIST1H1B antibody at 1.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 23 kDa. Observed band size: 32 kDa.
	Immunofluorescence staining of HepG2 cells with PACO56601 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	Chromatin Immunoprecipitation Hela (4*10⁶, treated with 100nM calyculin A for 60min) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1B (PACO56601) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta -Globin promoter.

Background:	Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Synonyms:	Histone H1.5 (Histone H1a) (Histone H1b) (Histone H1s-3), HIST1H1B, H1F5

UniProt Protein Function:	H1B: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. Belongs to the histone H1/H5 family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.1 Cellular Component: nuclear chromatin; nuclear heterochromatin; nucleosome Molecular Function:chromatin DNA binding; histone deacetylase binding Biological Process: establishment and/or maintenance of chromatin architecture; negative regulation of transcription from RNA polymerase II promoter; nucleosome assembly; positive regulation of cell growth; positive regulation of histone H3-K9 methylation; protein stabilization
NCBI Summary:	Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:	P16401
NCBI GenInfo Identifier:	19856407
NCBI Gene ID:	3009
NCBI Accession:	P16401.3
UniProt Secondary Accession:	P16401,Q14529, Q3MJ42,
UniProt Related Accession:	P16401
Molecular Weight:	22,580 Da
NCBI Full Name:	Histone H1.5
NCBI Synonym Full Names:	histone cluster 1, H1b
NCBI Official Symbol:	HIST1H1B
NCBI Official Synonym Symbols:	H1; H1B; H1.5; H1F5; H1s-3
NCBI Protein Information:	histone H1.5
UniProt Protein Name:	Histone H1.5
UniProt Synonym Protein Names:	Histone H1a; Histone H1b; Histone H1s-3
Protein Family:	Histone
UniProt Gene Name:	HIST1H1B
UniProt Entry Name:	H15_HUMAN