Phospho-EZH2/KMT6-T311 Antibody (CABP1269)

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SKU:
CABP1269
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Phospho-EZH2/KMT6-T311 Antibody (CABP1269)
Antibody SKU:CABP1269
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Rat
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around T311 of human EZH2/KMT6 (NP_001190176.1).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Rat
Positive Samples:C6
Immunogen:A synthetic phosphorylated peptide around T311 of human EZH2/KMT6 (NP_001190176.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KNTE T
Cellular Location:Nucleus
Calculated MW:79kDa/81kDa/84kDa/85kDa/86kDa
Observed MW:98KDa
Synonyms:ENX-1, ENX1, EZH1, EZH2b, KMT6, KMT6A, WVS, WVS2, EZH2, KMT6 / EZH2
Background:This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
Western blot analysis of extracts of C6 cells, using Phospho-EZH2/KMT6-T311 antibody at 1:1000 dilution. C6 cells were treated by AICAR (0.5 mM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.Western blot analysis of extracts of C6 cells, using Phospho-EZH2/KMT6-T311 antibody at 1:1000 dilution. C6 cells were treated by AICAR (0.5 mM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
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