Phospho-ERK/JNK/P38 alpha PharmaGenie ELISA Kit (SBRS1886)

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SKU:
SBRS1886
Product Type:
ELISA Kit
Reactivity:
Phospho-ERK
Reactivity:
JNK
Reactivity:
P38?
Applications:
ELISA
ELISA Type:
Sandwich

Description

Phospho-ERK/JNK/P38 alpha PharmaGenie ELISA Kit (SBRS1886)

Assay Genie MAPK Pathway ELISA Sample Kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated ERK1/2, JNK and P38α protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-ERK1/2, total ERK1/2, phospho-JNK, total JNK, phospho- P38α and total P38α. For each target, a capture antibody has been coated onto microwells. Samples are pipetted into the wells and target protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection antibody is used to detect the captured target protein. After washing away unbound antibody, an HRP-conjugated secondary antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of target protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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