Antibody Name:	Phospho-Cdc25b (Ser149) Antibody (PACO24346)
Antibody SKU:	PACO24346
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:	Mouse, Rat
Immunogen:	Peptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P) derived from Mouse Cdc25B.

Form:	Liquid
Storage Buffer:	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western blot analysis of extracts from 3T3 cells untreated(lane 1) or treated with Hydroxyurea(lane 2) using Cdc25B(Phospho-Ser149) Antibody.
	Western blot analysis of extracts from HT29 cells, treated with PMA or calf intestinal phosphatase (CIP), using Cdc25B (Phospho-Ser149) Antibody.

Background:

Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Li G, et al. (2010) Retrovirology.7:59.
Wang Z,et al. (2010) BMC Cancer.10:233.
Lucci MA,et al. (2010) Cell Oncol.32(5-6):361-72.

Synonyms:

CDC25B

UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q9DBN8
NCBI GenInfo Identifier:	161621261
NCBI Gene ID:	12531
NCBI Accession:	NP_001104545.1
UniProt Secondary Accession:	Q9DBN8
UniProt Related Accession:	Q9DBN8,P30306
Molecular Weight:	62,927 Da
NCBI Full Name:	M-phase inducer phosphatase 2 isoform b
NCBI Synonym Full Names:	cell division cycle 25B
NCBI Official Symbol:	Cdc25b
NCBI Official Synonym Symbols:	AI604853
NCBI Protein Information:	M-phase inducer phosphatase 2
UniProt Protein Name:	Cdc25b protein
UniProt Synonym Protein Names:	Cdc25b protein
Protein Family:
UniProt Gene Name:	Cdc25b
UniProt Entry Name:	Q9DBN8_MOUSE