Phospho-BRD4-S1083 Antibody (CABP1235)

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SKU:
CABP1235
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Phospho-BRD4-S1083 Antibody (CABP1235)
Antibody SKU:CABP1235
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around S1083 of human BRD4 (NP_490597.1).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa
Immunogen:A synthetic phosphorylated peptide around S1083 of human BRD4 (NP_490597.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HQSP P
Cellular Location:
Calculated MW:80kDa/88kDa/152kDa
Observed MW:200KDa
Synonyms:BRD4, CAP, HUNK1, HUNKI, MCAP, bromodomain containing 4
Background:The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Western blot analysis of extracts of various cell lines, using Phospho-BRD4-S1083 antibody at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. HeLa cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.Western blot analysis of extracts of various cell lines, using Phospho-BRD4-S1083 antibody at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. HeLa cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.
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