Name: Phospho-BLM (Thr99) Antibody (PACO24377)
SKU: PACO24377
Availability: OutOfStock

Antibody Name:	Phospho-BLM (Thr99) Antibody (PACO24377)
Antibody SKU:	PACO24377
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100
Species Reactivity:	Human
Immunogen:	Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome.

Form:	Liquid
Storage Buffer:	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody.The lane on the right is treated with the antigen-specific peptide.
	Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody (left)or the same antibody preincubated with blocking peptide (right).

Background:	Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).
Synonyms:	RECQ2; RECQL3; type 2; EC 3.6.1; RecQ protein-like 3

UniProt Protein Function:	BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.Protein type: DNA repair, damage; Helicase; DNA replication; EC 3.6.4.12Chromosomal Location of Human Ortholog: 15q26.1Cellular Component: chromosome, telomeric region; cytoplasm; lateral element; nuclear chromosome; nuclear matrix; nucleolus; nucleoplasm; nucleus; PML bodyMolecular Function: ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATP-dependent helicase activity; ATPase activity; bubble DNA binding; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; p53 binding; protein binding; single-stranded DNA bindingBiological Process: DNA double-strand break processing; DNA replication; DNA synthesis during DNA repair; double-strand break repair via homologous recombination; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of cell division; negative regulation of DNA recombination; positive regulation of transcription, DNA-dependent; protein oligomerization; protein sumoylation; regulation of cyclin-dependent protein kinase activity; replication fork processing; response to DNA damage stimulus; response to X-ray; strand displacementDisease: Bloom Syndrome
UniProt Protein Details:
NCBI Summary:	The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008]
UniProt Code:	P54132
NCBI GenInfo Identifier:	1705486
NCBI Gene ID:	641
NCBI Accession:	P54132.1
UniProt Secondary Accession:	P54132,Q52M96
UniProt Related Accession:	P54132
Molecular Weight:	159,000 Da
NCBI Full Name:	Bloom syndrome protein
NCBI Synonym Full Names:	Bloom syndrome RecQ like helicase
NCBI Official Symbol:	BLM
NCBI Official Synonym Symbols:	BS; RECQ2; RECQL2; RECQL3
NCBI Protein Information:	Bloom syndrome protein
UniProt Protein Name:	Bloom syndrome protein
UniProt Synonym Protein Names:	DNA helicase, RecQ-like type 2; RecQ2; RecQ protein-like 3
Protein Family:	Bloom syndrome protein
UniProt Gene Name:	BLM
UniProt Entry Name:	BLM_HUMAN