Phospho-Beclin 1-Ser93 Antibody (CABP1287)
- SKU:
- CABP1287
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Phospho-Beclin 1-Ser93 Antibody (CABP1287) |
| Antibody SKU: | CABP1287 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S93 of human Beclin 1 (NP_003757.1). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa |
| Immunogen: | A synthetic phosphorylated peptide around S93 of human Beclin 1 (NP_003757.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TESA N |
| Cellular Location: | |
| Calculated MW: | 51kDa |
| Observed MW: | 60KDa |
| Synonyms: | ATG6, VPS30, beclin1 |
| Background: | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. |
 | Western blot analysis of extracts of HeLa cells, using Phospho-Beclin 1-Ser93 antibody at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s. |
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