Antibody Name:	Phospho-Bad (Ser112) Antibody (PACO24247)
Antibody SKU:	PACO24247
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Peptide sequence around phosphorylation site of serine 112 (H-S-S(p)-Y-P) derived from Mouse BAD.

Form:	Liquid
Storage Buffer:	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western blot analysis of extracts from cos7 cells untreated or treated with EGF using BAD(Phospho-Ser112) Antibody.
	Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Phospho-Ser112) Antibody(left) or the same antibody preincubated with blocking peptide(right).

Background:

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Zhang B, et al. (2004). Mol Cell Biol.24 (14): 6205-6214.
Rice PL, et al. (2003). Cancer Res.63 (3): 616-620.
Wang XQ, et al. (2001). J Biol Chem.276 (48): 44504-44511.

Synonyms:

Bbc2

UniProt Protein Function:	BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.
UniProt Protein Details:	Protein type:Apoptosis Cellular Component: mitochondrial outer membrane; mitochondrion; membrane; cytoplasm; intracellular; cytosol Molecular Function:protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; protein kinase binding; lipid binding; protein phosphatase binding; protein phosphatase 2B binding Biological Process: positive regulation of proteolysis; apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; regulation of caspase activity; cellular process regulating host cell cycle in response to virus; glucose homeostasis; positive regulation of apoptosis by virus; regulation of apoptosis; pore complex biogenesis; positive regulation of glucokinase activity; caspase activation; release of cytochrome c from mitochondria; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; ATP metabolic process; glucose catabolic process; cell proliferation; positive regulation of T cell differentiation; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; positive regulation of epithelial cell proliferation
UniProt Code:	Q61337
NCBI GenInfo Identifier:	2493288
NCBI Gene ID:	12015
NCBI Accession:	Q61337.1
UniProt Related Accession:	Q61337
Molecular Weight:
NCBI Full Name:	Bcl2-associated agonist of cell death
NCBI Synonym Full Names:	BCL2-associated agonist of cell death
NCBI Official Symbol:	Bad
NCBI Official Synonym Symbols:	Bbc2; AI325008
NCBI Protein Information:	bcl2-associated agonist of cell death; bcl-2-binding component 6; bcl2 antagonist of cell death; bcl-xL/Bcl-2-associated death promoter
UniProt Protein Name:	Bcl2-associated agonist of cell death
UniProt Synonym Protein Names:	Bcl-2-binding component 6; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death
Protein Family:	Bcl2-associated agonist of cell death
UniProt Gene Name:	Bad
UniProt Entry Name:	BAD_MOUSE