Name: Phospho-ARHGAP35 (Tyr1105) Antibody (PACO23932)
SKU: PACO23932
Availability: OutOfStock

Antibody Name:	Phospho-ARHGAP35 (Tyr1105) Antibody (PACO23932)
Antibody SKU:	PACO23932
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Peptide sequence around phosphorylation site of Tyrosine 1105(N-I-Y(p)-S-V) derived from Human GRF-1.

Form:	Liquid
Storage Buffer:	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western blot analysis of extracts from Mouse brain tissue using GRF-1 (Phospho-Tyr1105) Antibody.The lane on the left is treated with the antigen-specific peptide.

Background:	Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.
Synonyms:	GRLF1, P190A, P190-A, p190RhoGAP, p190ARhoGAP

UniProt Protein Function:	GRF-1: the human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTP-ase activating protein (GAP). May also act as a tumor suppressor. Two splice variant isoforms have been described. Protein type: Motility/polarity/chemotaxis; GAPs, Rac/Rho; Transcription factor; GAPs; Tumor suppressorChromosomal Location of Human Ortholog: 19q13.3Cellular Component: cytosol; nucleus; actin cytoskeletonMolecular Function: GTP binding; DNA binding; transcription corepressor activityBiological Process: regulation of cell shape; axon guidance; regulation of small GTPase mediated signal transduction; camera-type eye development; negative regulation of Rho protein signal transduction; transcription, DNA-dependent; small GTPase mediated signal transduction; neural tube closure; forebrain development; negative regulation of vascular permeability; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
UniProt Protein Details:
NCBI Summary:	The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NRY4
NCBI GenInfo Identifier:	150417981
NCBI Gene ID:	2909
NCBI Accession:	NP_004482.4
UniProt Secondary Accession:	Q9NRY4,Q14452, Q9C0E1, A7E2A4
UniProt Related Accession:	Q9NRY4
Molecular Weight:	155kDa
NCBI Full Name:	rho GTPase-activating protein 35
NCBI Synonym Full Names:	Rho GTPase activating protein 35
NCBI Official Symbol:	ARHGAP35
NCBI Official Synonym Symbols:	GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
NCBI Protein Information:	rho GTPase-activating protein 35; rho GAP p190A; glucocorticoid receptor repression factor 1; glucocorticoid receptor DNA binding factor 1; glucocorticoid receptor DNA-binding factor 1
UniProt Protein Name:	Rho GTPase-activating protein 35
UniProt Synonym Protein Names:	Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A
Protein Family:
UniProt Gene Name:	ARHGAP35
UniProt Entry Name:	RHG35_HUMAN