Phospho-AMPKa1-T183/AMPKa2-T172 Antibody (CABP1283)
- SKU:
- CABP1283
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Phospho-AMPKa1-T183/AMPKa2-T172 Antibody (CABP1283) |
| Antibody SKU: | CABP1283 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around T183 of human AMPKa1/AMPKa2PRKAA1 (NP_006242.5). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | C6 |
| Immunogen: | A synthetic phosphorylated peptide around T183 of human AMPKa1/AMPKa2PRKAA1 (NP_006242.5). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LRTS C |
| Cellular Location: | |
| Calculated MW: | 64kDa/65kDa/62kDa |
| Observed MW: | 62KDa |
| Synonyms: | AMPKa1/AMPKa2 |
| Background: |
 | Western blot analysis of extracts of C6 celles, using Phospho-AMPKa1-T183/AMPKa2-T172 antibody at 1:1000 dilution. C6 cells were treated by AICAR (0. 5 mM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
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