Phospho-AKT/GSKbeta/mTor PharmaGenie ELISA Kit (SBRS1746)

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SKU:
SBRS1746
Product Type:
ELISA Kit
Applications:
ELISA
ELISA Type:
Sandwich

Description

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Phospho-AKT/GSKbeta/mTor PharmaGenie ELISA Kit (SBRS1746)

Assay Genie AKT Pathway ELISA Sample kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates. By determining phosphorylated AKT, GSK3β and mTOR protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse phospho-AKT, total AKT, phospho-GSKβ, total GSKβ, phospho-mTOR and total mTOR. For each target, a capture antibody has been coated onto microwells. Samples are pipetted into the wells and target protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection antibody is used to detect the captured target protein. After washing away unbound antibody, an HRP-conjugated secondary antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of target protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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