PHAX Antibody (PACO52738)
- SKU:
- PACO52738
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | PHAX Antibody (PACO52738) |
| Antibody SKU: | PACO52738 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Phosphorylated adapter RNA export protein (6-243AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: A549 whole cell lysate. All lanes: PHAX antibody at 2.8µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa. Observed band size: 45 kDa. |
 | Immunofluorescent analysis of Hela cells using PACO52738 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/β heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA. |
| Synonyms: | Phosphorylated adapter RNA export protein (RNA U small nuclear RNA export adapter protein), PHAX, RNUXA |
| UniProt Protein Function: | RNUXA: A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation- independent manner. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA. Belongs to the PHAX family.Protein type: RNA-binding; Nuclear importChromosomal Location of Human Ortholog: 5q23.2Cellular Component: centrosome; cytosol; intracellular membrane-bound organelle; nucleoplasm; nucleusMolecular Function: protein bindingBiological Process: nuclear export; snRNA export from nucleus; snRNA transcription from RNA polymerase II promoter |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9H814 |
| NCBI GenInfo Identifier: | 74752718 |
| NCBI Gene ID: | 51808 |
| NCBI Accession: | Q9H814.1 |
| UniProt Secondary Accession: | Q9H814,Q9H8W1 |
| UniProt Related Accession: | Q9H814 |
| Molecular Weight: | 44,403 Da |
| NCBI Full Name: | Phosphorylated adapter RNA export protein |
| NCBI Synonym Full Names: | phosphorylated adaptor for RNA export |
| NCBI Official Symbol: | PHAX |
| NCBI Official Synonym Symbols: | RNUXA |
| NCBI Protein Information: | phosphorylated adapter RNA export protein |
| UniProt Protein Name: | Phosphorylated adapter RNA export protein |
| UniProt Synonym Protein Names: | RNA U small nuclear RNA export adapter protein |
| Protein Family: | Phosphorylated adapter RNA export protein |
| UniProt Gene Name: | PHAX |
| UniProt Entry Name: | PHAX_HUMAN |
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