Hormone & Small Molecule ELISA Kits

PGE2 ELISA Kit

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SKU:
UNFI0032
Product Type:
ELISA Kit
Size:
96 Assays
Sensitivity:
18.75pg/ml
Range:
31.25-2000pg/ml
ELISA Type:
Competitive
Synonyms:
PGE2, Prostaglandin E2
Reactivity:
Universal

Description

PGE2 ELISA Kit

PGE2, or Prostaglandin E2, is a bioactive lipid molecule derived from arachidonic acid. It acts as a local mediator and plays a significant role in various physiological processes, including inflammation, pain, and immune modulation. The PGE2 ELISA Kit is a laboratory tool used to quantify the levels of PGE2 in biological samples and assist researchers and clinicians in studying the role of PGE2 in different biological processes, disease states, and therapeutic interventions, thereby aiding in the understanding and diagnosis of various conditions related to PGE2.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

PGE2 (Prostaglandin E2) ELISA Kit

Product Code:

UNFI0032

Size:

96 Assays

Alias:

PGE2, Prostaglandin E2

Detection Method:

Competitive ELISA, Coated with Antibody

Reactivity:

Universal

Sensitivity:

18.75pg/ml

Range:

31.25-2000pg/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of PGE2 and the recovery rates were calculated by comparing the measured value to the expected amount of PGE2 in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

87-97

93

EDTA plasma (n=5)

85-103

94

UFH plasma (n=5)

90-103

97

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PGE2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

94-105%

89-96%

86-99%

EDTA plasma (n=5)

87-97%

86-101%

83-99%

UFH Plasma (n=5)

91-92%

82-100%

81-100%

CV(%)

Intra Assay <8

Inter Assay <10

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

2-8°C/-20°C

Lyophilized Standard

2

2-8°C/-20°C

Sample/Standard Dlution Buffer

20ml

2-8°C

Biotin-labeled Antibody (Concentrated)

120ul

2-8°C (Protection from light)

Antibody Dilution Buffer

10ml

2-8°C

HRP-Streptavidin Conjugate (SABC)

120ul

2-8°C(Protect from light)

SABC Dilution Buffer

10ml

2-8°C

TMB Substrate

10ml

2-8°C (Protection from light)

Stop Solution

10ml

2-8°C

Wash Buffer (25X)

30ml

2-8°C

Plate Sealer

5

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