pfo Antibody (PACO50258)
- SKU:
- PACO50258
- Product Type:
- Antibody
- Reactivity:
- Bacteria
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | pfo Antibody (PACO50258) |
| Antibody SKU: | PACO50258 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000 |
| Species Reactivity: | Clostridium perfringens |
| Immunogen: | Recombinant Clostridium perfringens Perfringolysin O protein (29-500AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in Recombinant protein. All lanes: pfo antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56 kDa. Observed band size: 56 kDa.. |
| Background: | Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation. |
| Synonyms: | Perfringolysin O (Theta-toxin) (Thiol-activated cytolysin), pfo, pfoA pfoR |
| UniProt Protein Function: | Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation. |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P0C2E9 |
| NCBI GenInfo Identifier: | 18309145 |
| NCBI Gene ID: | 988404 |
| NCBI Accession: | NP_561079.1 |
| UniProt Secondary Accession: | P0C2E9,P19995 |
| UniProt Related Accession: | P0C2E9 |
| Molecular Weight: | 68.7 kDa |
| NCBI Full Name: | perfringolysin O |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | pfoA |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | perfringolysin O |
| UniProt Protein Name: | Perfringolysin O |
| UniProt Synonym Protein Names: | Theta-toxin; Thiol-activated cytolysin |
| Protein Family: | Perfringolysin |
| UniProt Gene Name: | pfo |
| UniProt Entry Name: | TACY_CLOPE |
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