Peroxidase Activity Assay Kit (BA0008)

Peroxidase Activity Assay Kit - Information

Assay Genie's peroxidase activity assay uses H₂O₂ and an electron donor dye that forms a pink color during the peroxidase reaction. The optical density (570nm) or fluorescence intensity (Ex/Em = 530/590nm) is a direct measure of the enzyme activity.

Applications

For quantitative determination of peroxidase activity.

Peroxidase Activity Assay Kit - Key Features

• Fast and sensitive. Use as little as 10 samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Peroxidase Activity Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 20 mL Stop Reagent: 12 mL Resorufin: 1.5 mL Dye Reagent: 60 uL 3% Stabilized H2O2: 100 uL
Kit Requires	Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, black 96-well plates and plate reader capable of either measuring absorbance between 560-590 nm or fluorescence at lex/em= 530/585 nm.
Method of Detection	OD570nm, or FL530/585nm
Detection Limit	OD, FL: 4, 0.8 U/L
Samples	Biological
Species	All
Protocol Length	20 min
Size	100 tests
Storage	Store the Assay Buffer, Dye Reagent and Resorufin at -20 °C. The 3% Stabilized H2O2 should be stored between -20C and 4C. The Stop Reagent can be stored at any temperature between -20C and room temperature.
Shelf Life	12 months

More Details

PEROXIDASES (EC number 1.11.1.x) catalyze the following oxidation-reduction reactions:ROOR' + electron donor (2 e-) + 2H⁺ ROH + R'OH.For many peroxidases the optimal substrate is hydrogen peroxide (H₂O₂), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plant, fungi and bacteria (cytochrome-c peroxidase like enzymes such a horseradish peroxidase). Simple, direct and automation-ready procedures for determining peroxidase activity find wide applications.