Pepsin Activity Assay Kit
- SKU:
- MAES0284
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Gastric, Intestinal Tissue
- Sensitivity:
- 0.63 U/mL
- Range:
- 0.63-94.98 U/mL
Description
| Product Name: | Pepsin Activity Assay Kit |
| Product Code: | MAES0284 |
| Sample Type: | Gastric, Intestinal Tissue |
| Assay Type: | Enzyme Activity |
| Detection Instrument: | Microplate reader (650-670 nm, optimum wavelength: 660 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 0.63 U/mL |
| Detection Range: | 0.63-94.98 U/mL |
| Inter-assay CV: | 4.8-5.6% |
| Intra-assay CV: | 1.3-2.2% |
Pepsin can catalyze substrate hydrolysis, and the hydrolysate reacts with the chromogenic agent under alkaline conditions to form a blue substance. The blue substance has a maximum absorption peak at 660 nm, and the color depth is proportional to the activity of pepsin. The enzyme activity can be calculated by OD value
| Storage: | -20°C |
| Valid Period: | 12 months |
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