PEAR1 Antibody (PACO56354)
- SKU:
- PACO56354
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | PEAR1 Antibody (PACO56354) |
| Antibody SKU: | PACO56354 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human, Rat, Mouse |
| Immunogen: | Recombinant Human Platelet endothelial aggregation receptor 1 protein (932-1037AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Rat liver tissue, Mouse kidney tissue. All lanes: PEAR1 antibody at 5.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 111 kDa. Observed band size: 111 kDa. |
 | Immunofluorescence staining of U251 cells with PACO56354 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO56354 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells. |
| Synonyms: | Platelet endothelial aggregation receptor 1 (hPEAR1) (Multiple epidermal growth factor-like domains protein 12) (Multiple EGF-like domains protein 12), PEAR1, MEGF12 |
| UniProt Protein Function: | PEAR1: When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells. Belongs to the MEGF family.Protein type: Receptor, misc.; Membrane protein, integralChromosomal Location of Human Ortholog: 1q23.1 |
| UniProt Protein Details: | |
| NCBI Summary: | PEAR1 is a platelet receptor that signals upon the formation of platelet-platelet contacts independent of platelet activation and secondary to platelet aggregation (Nanda et al., 2005 [PubMed 15851471]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q5VY43 |
| NCBI GenInfo Identifier: | 122937343 |
| NCBI Gene ID: | 375033 |
| NCBI Accession: | NP_001073940.1 |
| UniProt Secondary Accession: | Q5VY43,Q8TEK2 |
| UniProt Related Accession: | Q5VY43 |
| Molecular Weight: | 110,666 Da |
| NCBI Full Name: | platelet endothelial aggregation receptor 1 |
| NCBI Synonym Full Names: | platelet endothelial aggregation receptor 1 |
| NCBI Official Symbol: | PEAR1 |
| NCBI Official Synonym Symbols: | JEDI; MEGF12 |
| NCBI Protein Information: | platelet endothelial aggregation receptor 1 |
| UniProt Protein Name: | Platelet endothelial aggregation receptor 1 |
| UniProt Synonym Protein Names: | Multiple epidermal growth factor-like domains protein 12; Multiple EGF-like domains protein 12 |
| Protein Family: | Platelet endothelial aggregation receptor |
| UniProt Gene Name: | PEAR1 |
| UniProt Entry Name: | PEAR1_HUMAN |
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