PE/GenieFluor 594 Anti-Human CD45RO Antibody [UCHL1]
- SKU:
- AGEL3148
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Applications:
- FCM
- Reactivity:
- Human
- Host Species:
- Mouse
- Isotype:
- Mouse IgG2a, κ
- Clone:
- UCHL1
- Conjugation:
- PE/AF594
Description
| Product Name: | PE/GenieFluor 594 Anti-Human CD45RO Antibody [UCHL1] |
| Product Code: | AGEL3148 |
| Clone No: | UCHL1 |
| Reactivity: | Human |
| Host Species: | Mouse |
| Applications: | FCM |
| Isotype: | Mouse IgG2a, κ |
| Isotype Control: | PE/GenieFluor 594 Mouse IgG2a, κ Isotype Control [C1.18.4] (AGEL3463) |
| Conjugation: | PE/AF594 |
| Form: | Liquid |
| Fluorescent Label: | PE/AF594 is designed to be excited by the blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 620 nm (e.g., a 610/20 nm bandpass filter). |
| Recommended Use: | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
| Storage Buffer: | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
| Stability & Storage: | Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
| Shipping: | Biological ice pack at 4°C |
CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.
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