PE/Cyanine5 Anti-Mouse H-2 Antibody [M1/42] (AGEL2421)
- SKU:
- AGEL2421
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Applications:
- FCM
- Reactivity:
- Mouse
- Host Species:
- Rat
- Isotype:
- Rat IgG2a, κ
- Clone:
- M1/42
- Conjugation:
- PE/Cyanine5
Description
Product Name: | PE/Cyanine5 Anti-Mouse H-2 Antibody [M1/42] (AGEL2421) |
Product Code: | AGEL2421 |
Clone No: | M1/42 |
Synonyms: | Mouse major histocompatibility complex (MHC) H-2, MHC I |
Reactivity: | Mouse |
Host Species: | Rat |
Applications: | FCM |
Isotype: | Rat IgG2a, κ |
Isotype Control: | PE/Cyanine5 Rat IgG2b, κ Isotype Control [LTF-2] (AGEL2669) |
Conjugation: | PE/Cyanine5 |
Form: | Liquid |
Fluorescent Label: | PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter). |
Recommended Use: | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Storage Buffer: | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Stability & Storage: | Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Shipping: | Biological ice pack at 4°C |
The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981). MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.