Oxaloacetate Colorimetric Assay Kit
- SKU:
- MAES0273
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Animal Tissue
- Sensitivity:
- 0.017 mmol/L
- Range:
- 0.017-1 mmol/L
- Assay Time:
- 15 min
Description
| Product Name: | Oxaloacetate Colorimetric Assay Kit |
| Product Code: | MAES0273 |
| Sample Type: | Animal Tissue |
| Assay Time: | 15 min |
| Assay Type: | Quantitative |
| Detection Instrument: | Microplate reader (402-422 nm, optimum wavelength: 412 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 0.017 mmol/L |
| Detection Range: | 0.017-1 mmol/L |
| Inter-assay CV: | 0.023 |
| Intra-assay CV: | 0.021 |
Oxaloacetic acid (OAA), one of the intermediates in the tricarboxylic acid cycle is an important substance in carbon and nitrogen metabolism. Oxaloacetate reacts with substrate acetyl-coA under the action of enzyme. The substance produced can react with DTNB, which has the maximum absorption peak at 412 nm. The content of oxaloacetate can be determined by measuring the absorbance value.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Incubator, Ultrafiltration tube (50kD) |
| Other Instruments Required: | Normal saline (0.9% NaCl) |
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