Antibody Name:	Outer capsid protein VP4 Antibody (PACO34198)
Antibody SKU:	PACO34198
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA
Recommended Dilutions:
Species Reactivity:	Rotavirus A
Immunogen:	Recombinant Rotavirus A Outer capsid protein VP4 protein (247-479AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Background:

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. According to the considered strain, VP4 seems to essentially target sialic acid, and/or the integrin heterodimer ITGA2/ITGB1 (By similarity).By similarity Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment (By similarity).By similarity VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid, dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact (By similarity).

Synonyms:

Outer capsid protein VP4 (Hemagglutinin) [Cleaved into: Outer capsid protein VP8*; Outer capsid protein VP5*]

UniProt Protein Function:	Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. According to the considered strain, VP4 seems to essentially target sialic acid and/or the integrin heterodimer ITGA2/ITGB1 ().
UniProt Protein Details:
NCBI Summary:
UniProt Code:	P11200
NCBI GenInfo Identifier:	226693610
NCBI Gene ID:
NCBI Accession:	P11200.3
UniProt Secondary Accession:	P11200,Q86201, B3SRW7
UniProt Related Accession:	P11200
Molecular Weight:
NCBI Full Name:	Outer capsid protein VP4
NCBI Synonym Full Names:
NCBI Official Symbol:
NCBI Official Synonym Symbols:
NCBI Protein Information:
UniProt Protein Name:	Outer capsid protein VP4
UniProt Synonym Protein Names:	Hemagglutinin
Protein Family:
UniProt Gene Name:
UniProt Entry Name:	VP4_ROTHT