OTOP1 Antibody (PACO60152)
- SKU:
- PACO60152
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | OTOP1 Antibody (PACO60152) |
| Antibody SKU: | PACO60152 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Proton channel OTOP1 protein (414-544AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO60152 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO60152 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Required for normal formation of otoconia in the inner ear. Inhibits P2Y purinoceptors. Modulates calcium homeostasis and influx of calcium in response to extracellular ATP (By similarity). |
| Synonyms: | Otopetrin-1, OTOP1 |
| UniProt Protein Function: | OTOP1: Required for normal formation of otoconia in the inner ear. Inhibits P2Y purinoceptors. Modulates calcium homeostasis and influx of calcium in response to extracellular ATP. Belongs to the otopetrin family.Protein type: Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 4p16.3 |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q7RTM1 |
| NCBI GenInfo Identifier: | 74713136 |
| NCBI Gene ID: | 133060 |
| NCBI Accession: | Q7RTM1.1 |
| UniProt Secondary Accession: | Q7RTM1,A1L476 |
| UniProt Related Accession: | Q7RTM1 |
| Molecular Weight: | 67,353 Da |
| NCBI Full Name: | Otopetrin-1 |
| NCBI Synonym Full Names: | otopetrin 1 |
| NCBI Official Symbol: | OTOP1 |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | otopetrin-1 |
| UniProt Protein Name: | Otopetrin-1 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Otopetrin |
| UniProt Gene Name: | OTOP1 |
| UniProt Entry Name: | OTOP1_HUMAN |
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