Ostreolysin Recombinant Protein (RPPB4173)
- SKU:
- RPPB4173
- Product Type:
- Recombinant Protein
- Uniprot:
- Q56QW9
Description
| Product Name: | Ostreolysin Recombinant Protein |
| Product Code: | RPPB4173 |
| Size: | 20µg |
| Target: | Ostreolysin |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The Ostreolysin protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3. |
| Solubility: | It is recommended to reconstitute the lyophilized Ostreolysin in sterile 0.4% NaHCO3, not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Pleurotus Ostreatus Ostreolysin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon C between 2-7 days and for future use reconstituted Ostreolysin should be stored at 4°C below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by:(a) Analysis by Gel filtration analysis.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The N-terminal amino sequence is Ala-Tyr-Ala-Gln-Trp-Val� |
| Biological Activity: | Ostreolysin has potent anti-carcinogenic activity in several colon cancer cell lines.� |
| UniProt Code: | Q56QW9 |
Ostreolysin is extracted from Pleurotus ostreatus (oyster mushroom). It is a pore forming protein, which contains a lytic part to both cholesterol and sphingomyelin containing membranes. Because of their cholesterol content and the appearance of ostreolysin in the detergent resistant membranes, ostreolysin is cytotoxic towards the ovary cells of Chinese hamster.� It seems that Ostreolysin spots a rich lipid cholesterol phase, presumably the liquid ordered phase.
Pleurotus Ostreatus Ostreolysin Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 137 amino acids and having a molecular mass of 15 kDa.The Ostreolysin is purified by proprietary chromatographic techniques.
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