Cell Death Recombinant Proteins

Osteoprotegerin/TNFRSF11B Recombinant Protein (RPES0670)

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RPES0670

Description

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Osteoprotegerin/TNFRSF11B Recombinant Protein

TNFRSF11B is a secreted protein; containing 2 death domains and 4 TNFR-Cys repeats. TNFRSF11B is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL). By binding RANKL; TNFRSF11B inhibits nuclear kappa B (NF-?B) which is a central and rapid acting transcription factor for immune-related genes; and a key regulator of inflammation; innate immunity; and cell survival and differentiation. TNFRSF11B levels are influenced by voltage-dependent calcium channelsCav1.2. TNFRSF11B can reduce the production of osteoclasts by inhibiting the differentiation of osteoclast precursors (osteoclasts are related to monocytes/macrophages and are derived from granulocyte/macrophage-forming colony units (CFU-GM)) into osteoclasts and also regulates the resorption of osteoclasts in vitroand in vivo. TNFRSF11B binding to RANKL on osteoblast/stromal cells; blocks the RANKL-RANK ligand interaction between osteoblast/stromal cells and osteoclast precursors. This has the effect of inhibiting the differentiation of the osteoclast precursor into a mature osteoclast.

Product Name:Osteoprotegerin/TNFRSF11B Recombinant Protein (RPES0670)
Product Code:RPES0670
Size:10µg
Species:Human
Expressed Host:Human Cells
Synonyms:Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin, TNFRSF11B, OCIF, OPG,PDB5,TR1
Accession:O00300
Sequence:Glu22-Leu401
Fusion tag:C-His
Activity:Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L?929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 10.6 ng/ml.
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:Recombinant Human Osteoprotegerin is produced by our Mammalian expression system and the target gene encoding Glu22-Leu401 is expressed with a 6His tag at the C-terminus.
Purity:> 95% as determined by reducing SDS-PAGE.
Mol Mass:44.65 kDa
AP Mol Mass:57 kDa
Formulation:Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.4.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Research Area:
Cell Death
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