Cell Death Recombinant Proteins
Osteoprotegerin/TNFRSF11B Recombinant Protein (RPES0670)
- SKU:
- RPES0670
- Product Type:
- Recombinant Protein
- Species:
- Human
- Research Area:
- Cell Death
Description
Osteoprotegerin/TNFRSF11B Recombinant Protein
TNFRSF11B is a secreted protein; containing 2 death domains and 4 TNFR-Cys repeats. TNFRSF11B is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL). By binding RANKL; TNFRSF11B inhibits nuclear kappa B (NF-?B) which is a central and rapid acting transcription factor for immune-related genes; and a key regulator of inflammation; innate immunity; and cell survival and differentiation. TNFRSF11B levels are influenced by voltage-dependent calcium channelsCav1.2. TNFRSF11B can reduce the production of osteoclasts by inhibiting the differentiation of osteoclast precursors (osteoclasts are related to monocytes/macrophages and are derived from granulocyte/macrophage-forming colony units (CFU-GM)) into osteoclasts and also regulates the resorption of osteoclasts in vitroand in vivo. TNFRSF11B binding to RANKL on osteoblast/stromal cells; blocks the RANKL-RANK ligand interaction between osteoblast/stromal cells and osteoclast precursors. This has the effect of inhibiting the differentiation of the osteoclast precursor into a mature osteoclast.
| Product Name: | Osteoprotegerin/TNFRSF11B Recombinant Protein (RPES0670) |
| Product Code: | RPES0670 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | Human Cells |
| Synonyms: | Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin, TNFRSF11B, OCIF, OPG,PDB5,TR1 |
| Accession: | O00300 |
| Sequence: | Glu22-Leu401 |
| Fusion tag: | C-His |
| Activity: | Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L?929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 10.6 ng/ml. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Osteoprotegerin is produced by our Mammalian expression system and the target gene encoding Glu22-Leu401 is expressed with a 6His tag at the C-terminus. |
| Purity: | > 95% as determined by reducing SDS-PAGE. |
| Mol Mass: | 44.65 kDa |
| AP Mol Mass: | 57 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.4. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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