OSBPL7 Antibody (PACO63487)
- SKU:
- PACO63487
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | OSBPL7 Antibody (PACO63487) |
| Antibody SKU: | PACO63487 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Oxysterol-binding protein-related protein 7 protein (1-249AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO63487 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO63487 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | autophagosome, cytosol, perinuclear endoplasmic reticulum, plasma membrane, cholesterol binding, sterol transporter activity, bile acid, biosynthetic process, cellular response to cholesterol, positive regulation of proteasomal protein catabolic process, regulation of autophagy |
| Synonyms: | Oxysterol-binding protein-related protein 7 (ORP-7) (OSBP-related protein 7), OSBPL7, ORP7 |
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Two transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9BZF2 |
| NCBI GenInfo Identifier: | 22035616 |
| NCBI Gene ID: | 114881 |
| NCBI Accession: | NP_665741.1 |
| UniProt Secondary Accession: | Q9BZF2,Q6PIV6, D3DTT6 |
| UniProt Related Accession: | Q9BZF2 |
| Molecular Weight: | 40,186 Da |
| NCBI Full Name: | oxysterol-binding protein-related protein 7 |
| NCBI Synonym Full Names: | oxysterol binding protein like 7 |
| NCBI Official Symbol: | OSBPL7 |
| NCBI Official Synonym Symbols: | ORP7 |
| NCBI Protein Information: | oxysterol-binding protein-related protein 7 |
| UniProt Protein Name: | Oxysterol-binding protein-related protein 7 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Oxysterol-binding protein-related protein |
| UniProt Gene Name: | OSBPL7 |
| UniProt Entry Name: |
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