Antibody Name:	ORF1 Antibody (PACO34358)
Antibody SKU:	PACO34358
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA
Recommended Dilutions:
Species Reactivity:	Hepatitis E virus genotype 1
Immunogen:	Recombinant Hepatitis E virus genotype 1 Non-structural polyprotein pORF1 protein (60-240AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Background:

Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m7GTP or m7GDP. GMP, GpppG, and GpppA were poor substrates for the methyltransferase. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m7GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure. Cap analogs such as m7GTP, m7GDP, et2m7GMP, and m2et7GMP inhibit the methyltransferase reaction (By similarity).

Synonyms:

Non-structural polyprotein pORF1 [Includes: Methyltransferase (EC 2.1.1.-) (EC 2.7.7.-); Putative papain-like cysteine protease (PLP) (EC 3.4.22.-); NTPase/helicase (EC 3.6.4.-); RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48)]

UniProt Protein Function:	Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m7GTP or m7GDP. GMP, GpppG, and GpppA were poor substrates for the methyltransferase. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m7GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure. Cap analogs such as m7GTP, m7GDP, et2m7GMP, and m2et7GMP inhibit the methyltransferase reaction ().
UniProt Protein Details:
NCBI Summary:
UniProt Code:	P33424
NCBI GenInfo Identifier:	4033773
NCBI Gene ID:
NCBI Accession:	P33424.2
UniProt Secondary Accession:	P33424,Q8UYD6
UniProt Related Accession:	P33424
Molecular Weight:	24.4 kDa
NCBI Full Name:	Non-structural polyprotein pORF1
NCBI Synonym Full Names:
NCBI Official Symbol:
NCBI Official Synonym Symbols:
NCBI Protein Information:
UniProt Protein Name:	Non-structural polyprotein pORF1
UniProt Synonym Protein Names:
Protein Family:	Protein
UniProt Gene Name:	ORF1
UniProt Entry Name:	POLN_HEVPA