NRF1 Transcription Factor Activity Assay
- SKU:
- TFAB00144
- Product Type:
- ELISA Kit
- ELISA Type:
- Transcription Factor Assay
- Analytes:
- NRF1
- Reactivity:
- Human
Description
| Product Name: | NRF1 Transcription Factor Activity Assay |
| Product Code: | TFAB00144 |
| Target: | NRF1 |
| Synonyms: | Alpha palindromic-binding protein, Alpha-pal |
| Reactivity: | Human |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie NRF1 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | NRF1: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Belongs to the NRF1/Ewg family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 7q32 Cellular Component: nucleoplasm; cytoplasm Biological Process: regulation of transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; generation of precursor metabolites and energy; organ regeneration; transcription, DNA-dependent; response to folic acid; organelle organization and biogenesis; response to hypoxia; response to lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; response to electrical stimulus; response to estradiol stimulus |
| NCBI Summary: | This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014] |
| UniProt Code: | Q16656 |
| NCBI GenInfo Identifier: | 12643732 |
| NCBI Gene ID: | 4899 |
| NCBI Accession: | Q16656.1 |
| UniProt Secondary Accession: | Q16656,Q15305, Q96AN2, A8K4C6, B4DDV6, |
| UniProt Related Accession: | Q16656 |
| Molecular Weight: | 55,674 Da |
| NCBI Full Name: | Nuclear respiratory factor 1 |
| NCBI Synonym Full Names: | nuclear respiratory factor 1 |
| NCBI Official Symbol: | NRF1 |
| NCBI Official Synonym Symbols: | ALPHA-PAL |
| NCBI Protein Information: | nuclear respiratory factor 1; alpha palindromic-binding protein |
| UniProt Protein Name: | Nuclear respiratory factor 1 |
| UniProt Synonym Protein Names: | Alpha palindromic-binding protein; Alpha-pal |
| Protein Family: | Nuclear respiratory factor |
| UniProt Gene Name: | NRF1 |
| UniProt Entry Name: | NRF1_HUMAN |
