Product Name:	NR2F6 Transcription Factor Activity Assay
Product Code:	TFAB00143
Target:	NR2F6
Reactivity:	Human, Mouse
Sample Types:	Nuclear or cell lysates

The Assay Genie NR2F6 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:	4.5 hours
Detection Method:	Colorimetric 450 nm
Size:	12 x 8-Well Microstrips
Storage:	4°C for 6 months

UniProt Protein Function:	EAR-2: Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type- specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC). Belongs to the nuclear hormone receptor family. NR2 subfamily.
UniProt Protein Details:	Protein type:Nuclear receptor; DNA-binding Chromosomal Location of Human Ortholog: 19p13.1 Cellular Component: nucleoplasm; nucleus Molecular Function:DNA binding; ligand-dependent nuclear receptor activity; protein binding; sequence-specific DNA binding; thyroid hormone receptor activity; transcription factor activity Biological Process: negative regulation of transcription from RNA polymerase II promoter; signal transduction; transcription initiation from RNA polymerase II promoter
UniProt Code:	P10588
NCBI GenInfo Identifier:	23503053
NCBI Gene ID:	2063
NCBI Accession:	P10588.2
UniProt Secondary Accession:	P10588,Q5XGA0, Q6P586, Q9BUE8, B2RC68,
UniProt Related Accession:	P10588
Molecular Weight:	42,979 Da
NCBI Full Name:	Nuclear receptor subfamily 2 group F member 6
NCBI Synonym Full Names:	nuclear receptor subfamily 2 group F member 6
NCBI Official Symbol:	NR2F6
NCBI Official Synonym Symbols:	EAR2; EAR-2; ERBAL2
NCBI Protein Information:	nuclear receptor subfamily 2 group F member 6
UniProt Protein Name:	Nuclear receptor subfamily 2 group F member 6
UniProt Synonym Protein Names:	V-erbA-related protein 2; EAR-2
Protein Family:	Nuclear receptor subfamily
UniProt Gene Name:	NR2F6
UniProt Entry Name:	NR2F6_HUMAN