Product Name:	NR1I3 Transcription Factor Activity Assay
Product Code:	TFAB00178
Target:	NR1I3
Synonyms:	Constitutive activator of retinoid response, Constitutive active response, Constitutive androstane receptor, CAR, Orphan nuclear receptor MB67
Reactivity:	Human
Sample Types:	Nuclear or cell lysates

The Assay Genie NR1I3 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:	4.5 hours
Detection Method:	Colorimetric 450 nm
Size:	12 x 8-Well Microstrips
Storage:	4°C for 6 months

UniProt Protein Function:	NR1I3: Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element. Interacts with ECT2. Heterodimer of NR1I3 and RXR. Interacts with PSMC4. Directly interacts with DNAJC7. The DNAJC7-NR1I3 complex may also include HSP90. By dexamethasone. Predominantly expressed in liver. Belongs to the nuclear hormone receptor family. NR1 subfamily. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor; DNA-binding Chromosomal Location of Human Ortholog: 1q23.3 Cellular Component: nucleoplasm; cytoskeleton; cytoplasm; cytosol; nucleus Molecular Function:ligand-dependent nuclear receptor activity; androgen receptor activity; DNA binding; zinc ion binding; transcription coactivator activity; thyroid hormone receptor activity; transcription factor activity Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; androgen receptor signaling pathway; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q14994
NCBI GenInfo Identifier:	49066046
NCBI Gene ID:	9970
NCBI Accession:	Q14994.2
UniProt Secondary Accession:	Q14994,Q0VAC9, Q4U0F0, E9PB75, E9PC13, E9PDU3, E9PGH6 E9PH10, E9PHC8, E9PHN4, F1D8Q0, F1D8Q1,
UniProt Related Accession:	Q14994
Molecular Weight:	352
NCBI Full Name:	Nuclear receptor subfamily 1 group I member 3
NCBI Synonym Full Names:	nuclear receptor subfamily 1, group I, member 3
NCBI Official Symbol:	NR1I3
NCBI Official Synonym Symbols:	CAR; CAR1; MB67
NCBI Protein Information:	nuclear receptor subfamily 1 group I member 3; constitutive active receptor; constitutive active response; orphan nuclear receptor MB67; orphan nuclear hormone receptor; constitutive androstane receptor; constitutive activator of retinoid response; constitutive androstane nuclear receptor variant 2; constitutive androstane nuclear receptor variant 3; constitutive androstane nuclear receptor variant 4; constitutive androstane nuclear receptor variant 5
UniProt Protein Name:	Nuclear receptor subfamily 1 group I member 3
UniProt Synonym Protein Names:	Constitutive activator of retinoid response; Constitutive active response; Constitutive androstane receptor; CAR; Orphan nuclear receptor MB67
Protein Family:	Nuclear receptor subfamily
UniProt Gene Name:	NR1I3
UniProt Entry Name:	NR1I3_HUMAN