NPRL3 Antibody (PACO60965)
- SKU:
- PACO60965
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | NPRL3 Antibody (PACO60965) |
| Antibody SKU: | PACO60965 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human GATOR complex protein NPRL3 protein (349-482AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO60965 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Western Blot. Positive WB detected in: U87 whole cell lysate. All lanes: NPRL3 antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 64 kDa. Observed band size: 64 kDa. |
| Background: | As a component of the GATOR1 complex functions as an inhibitor of the amino acid, sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid, sensing branch of the TORC1 pathway. |
| Synonyms: | GATOR complex protein NPRL3 (-14 gene protein) (Alpha-globin regulatory element-containing gene protein) (Nitrogen permease regulator 3-like protein) (Protein CGTHBA), NPRL3, C16orf35 CGTHBA MARE |
| UniProt Protein Function: | NPRL3: The function of the encoded protein is not known. [provided by RefSeq, Aug 2011]Protein type: Unknown functionChromosomal Location of Human Ortholog: 16p13.3Molecular Function: GTPase activator activityBiological Process: cellular response to amino acid starvation; negative regulation of TOR signaling pathway |
| UniProt Protein Details: | |
| NCBI Summary: | The function of the encoded protein is not known. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q12980 |
| NCBI GenInfo Identifier: | 18202492 |
| NCBI Gene ID: | 8131 |
| NCBI Accession: | Q12980.1 |
| UniProt Secondary Accession: | Q12980,Q1W6H0, Q4TT56, Q92469, D3DU40 |
| UniProt Related Accession: | Q12980 |
| Molecular Weight: | 63,605 Da |
| NCBI Full Name: | Nitrogen permease regulator 3-like protein |
| NCBI Synonym Full Names: | NPR3 like, GATOR1 complex subunit |
| NCBI Official Symbol: | NPRL3 |
| NCBI Official Synonym Symbols: | MARE; NPR3; HS-40; RMD11; CGTHBA; C16orf35 |
| NCBI Protein Information: | nitrogen permease regulator 3-like protein |
| UniProt Protein Name: | Nitrogen permease regulator 3-like protein |
| UniProt Synonym Protein Names: | -14 gene protein; Alpha-globin regulatory element-containing gene protein; Protein CGTHBA |
| Protein Family: | |
| UniProt Gene Name: | NPRL3 |
| UniProt Entry Name: | NPRL3_HUMAN |
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