Signaling Pathway Assays
NOS Activity Assay Kit (Colorimetric) (BA0138)
- SKU:
- BA0138
- Product Type:
- Assay
- Research Area:
- Metabolism
Description
NOS Activity Assay Kit
Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase (NOS), is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Simple, direct and non-radioactive procedures for measuring NOS are becoming popular in Research and Drug Discovery.
Assay Genie's colorimetric Nitric Oxide Synthase (NOS) Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 40 min
Applications
For quantitative determination of nitric oxide synthase (NOS) activity and screen for NOS inhibitor.
Key Features
- Sensitive and accurate. Detection range 0.25 - 25 U/L in 96-well plate.
- Rapid and reliable. Can be completed in 40 min if reduction of NO3- to NO2- is performed at 60°C.
NOS Activity Assay Kit Information:
| Kit Includes: | Assay Buffer: 10 mL, Substrate: 600 mL, GDH: 120 mL, Reagent A: 12 mL, Reagent B: 500 mL, Reagent C: 12 mL, Reagent D: 600 mL, Reagent E: 1.5 mL, ZnSO4: 1 mL, Standard: 1 mL, NaOH: 1 mL |
| Kit Requires: | Pipetting devices, eppendorf tubes, eppendorf centrifuge, clear, flat bottomed 96 well plates or cuvettes, plate reader or spectrophotometer and heat block or hot water bath (optional). |
| Method of Detection: | OD540nm |
| Detection Limit: | 0.25 U/L |
| Samples: | Biological |
| Species: | All |
| Protocol Length: | 40 min |
| Size: | 100 tests |
| Storage: | Store Assay Buffer, Substrate, Reagent D, Reagent E and GDH at -20°C. Store all other reagents at 4°C. |
| Shelf Life: | 6 months |
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