Name: NIT2 Antibody (PACO18757)
SKU: PACO18757
Availability: OutOfStock

Antibody Name:	NIT2 Antibody (PACO18757)
Antibody SKU:	PACO18757
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:50-1:200
Species Reactivity:	Human, Mouse
Immunogen:	Synthetic peptide of human NIT2

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PACO18757(NIT2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
	Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Mouse liver tissue, Mouse kidney tissue, RAW264.7 cells, Primary antibody: PACO18757(NIT2 Antibody) at dilution 1/950, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 90 seconds.
	The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO18757(NIT2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).

Background:	Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.
Synonyms:	Itrilase family, member 2

UniProt Protein Function:	NIT2: a protein of unknown function. A CN hydrolase domain suggests that this protein may be involved in nitrilase-related metabolism and handling.
UniProt Protein Details:	Protein type:EC 3.5.1.3; Hydrolase; Mitochondrial Chromosomal Location of Human Ortholog: 3q12.2 Cellular Component: centrosome; mitochondrion; cytoplasm Molecular Function:omega-amidase activity Biological Process: oxaloacetate metabolic process; glutamine metabolic process; asparagine metabolic process
UniProt Code:	Q9NQR4
NCBI GenInfo Identifier:	74725271
NCBI Gene ID:	56954
NCBI Accession:	Q9NQR4.1
UniProt Secondary Accession:	Q9NQR4,Q8WUF0, B2R9A3, D3DN47,
UniProt Related Accession:	Q9NQR4
Molecular Weight:	276
NCBI Full Name:	Omega-amidase NIT2
NCBI Synonym Full Names:	nitrilase family, member 2
NCBI Official Symbol:	NIT2
NCBI Official Synonym Symbols:	HEL-S-8a
NCBI Protein Information:	omega-amidase NIT2; Nit protein 2; nitrilase homolog 2; epididymis secretory sperm binding protein Li 8a
UniProt Protein Name:	Omega-amidase NIT2
UniProt Synonym Protein Names:	Nitrilase homolog 2
Protein Family:	Nitrilase
UniProt Gene Name:	NIT2
UniProt Entry Name:	NIT2_HUMAN